The zebrafish is one of the primary models of the HRP; hair cells in its lateral line organ show robust regeneration, the accessibility of this organ means that we can watch the whole process take place using microscopy. However, the lack of complete synchronization of this process means that when we are looking at molecular changes, they are smeared out in time. This project will use single-cell transcript analysis techniques to order supporting cell responses to damage and determine the order of expression of transcripts with high precision.